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Ferreira Neto,José Mauricio; Ferreira,Fernanda Pinto; Miura,Ana Carolina; Almeida,Jonatas Campos de; Martins,Felippe Danyel Cardoso; Souza,Marielen de; Bronkhorst,Dalton Evert; Romanelli,Paulo Roberto; Pasquali,Aline Kuhn Sbruzzi; Santos,Hannah Lia Ettiene Peruch Lemos dos; Benitez,Aline do Nascimento; Caldart,Eloiza Teles; Zanella,Luiz Francisco; Freire,Roberta Lemos; Navarro,Italmar Teodorico. |
ABSTRACT: The present study aimed to investigate an abortion outbreak in a dairy goat herd in the municipality of Arapoti, Parana, Brazil. At the beginning of the outbreak, blood samples were collected from 33 goats with clinical signs; later, of the whole goat herd, two cats and two dogs. Milk samples were collected from 78 lactating goats. Four environmental soil samples and four samples of feed residue from goat feeders were collected too. Immunofluorescence antibody test (IFA) was used for serodiagnosis, the molecular analysis was conducted by means of the polymerase chain reaction (PCR), for the isolation of the etiological agent the bioassay was used. The results of the IFA revealed that 76.53% (137/179) of the goats, two dogs and two cats were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma; Goats. IFA; PCR; Bioassay.. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500500 |
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Araújo,Flábio Ribeiro de; Sarti,Enio Cesar; Crocci,Adalberto José; Seabra,Viviane Maria Souza; Amorim,Jan Heber; Cusinato,Flávia Queiróz; Araújo,Cristina Pires de; Carvalho,Cristiano Marcelo Espinola. |
Amostras de soro obtidas de estudantes do curso de Medicina Veteriná ria da Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Campo Grande, MS, Brasil, foram examinadas para a presença de anticorpos contra Toxoplasma gondii. Dos 145 soros testados, 44 (30,34%) foram positivos na hemaglutinação, com título igual ou superior a 1:16. Não foram observadas associações entre as caracterí sticas epidemiológicas examinadas, tais como hábitos alimentares (ingestão de carne bovina crua ou malpassada, vegetais crus/não lavados, produtos lácteos não pasteurizados) ou contato constante com cães e a presença de anticorpos contra T. gondii, exceto pelo percentual significativamente maior de estudantes soropositivos que relataram ter contato freqüente... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma; Levantamento sorológico; Estudantes; Medicina veterinária. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000600016 |
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Yoda, Tomoko; Sakurai, Haruhisa; Igarashi, Ikuo; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; 依田, 知子; 櫻井, 治久; 五十嵐, 郁男; 小俣, 吉孝; 齊藤, 篤志; 鈴木, 直義. |
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Palavras-chave: Toxoplasma; Serum components; Bovine; Toxoplasma lysate antigen; Lymphokines. |
Ano: 1990 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1569 |
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Vallochi,A.L.; Yamamoto,J.H.; Schlesinger,D.; Machado,M.A.C.; Silveira,C.; Martins,M.C.; Belfort Jr.,R.; Kalil,J.; Rizzo,L.V.. |
Toxoplasma gondii is an obligatory intracellular parasite whose life cycle may include man as an intermediate host. More than 500 million people are infected with this parasite worldwide. It has been previously reported that T. gondii contains a superantigen activity. The purpose of the present study was to determine if the putative superantigen activity of T. gondii would manifest towards human T cells. Peripheral blood mononuclear cells (PBMC) from individuals with no previous contact with the parasite were evaluated for proliferation as well as specific Vß expansion after exposure to Toxoplasma antigens. Likewise, PBMC from individuals with the congenital infection were evaluated for putative Vß family deletions in their T cell repertoire. We also... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma; Tolerance; Superantigen; T cell receptor. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800008 |
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